In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam.  With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results.  RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation.  In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay.  For more information and pricing, please click here. 

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents.  MFI² represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available.   By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results.  Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI², please visit the Serology section of this site.

Myles MH, Livingston RS, Franklin CL. Pathogenicity of Helicobacter rodentium in A/JCr and SCID mice. Comp Med. 2004 Oct;54(5):549-57.

Livingston RS, Myles MH, Livingston BA, Criley JM, Franklin CL. Sex influence on chronic intestinal inflammation in Helicobacter hepaticus-infected A/JCr mice. Comp Med. 2004 Jun;54(3):301-8.

Myles MH, Livingston RS, Livingston BA, Criley JM, Franklin CL. Analysis of gene expression in ceca of Helicobacter hepaticus-infected A/JCr mice before and after development of typhlitis. Infect Immun. 2003 Jul;71(7):3885-93.

Drazenovich NL, Franklin CL, Livingston RS, Besselsen DG. Detection of rodent Helicobacter spp. by use of fluorogenic nuclease polymerase chain reaction assays. Comp Med. 2002 Aug;52(4):347-53.

Franklin CL, Gorelick PL, Riley LK, Dewhirst FE, Livingston RS, Ward JM, Beckwith CS, Fox JG. Helicobacter typhlonius sp. nov., a Novel Murine Urease-Negative Helicobacter Species. J Clin Microbiol. 2001 Nov;39(11):3920-6.

Franklin CL. Microbial considerations in genetically engineered mouse research. ILAR J. 2006;47(2):141-55.

Spier AW, Toth LA, Faingold CA, Franklin CL. Clinical patent ductus arteriosus in adult genetically epilepsy-prone rats. Comp Med. 2005 Feb;55(1):85-91

Pfeiffer BJ, Franklin CL, Hsieh FH, Bank RA, Phillips CL. Alpha 2(I) collagen deficient oim mice have altered biomechanical integrity, collagen content, and collagen crosslinking of their thoracic aorta. Matrix Biol. 2005 Oct;24(7):451-8. Epub 2005 Aug 10.

Clarke LL, Gawenis LR, Bradford EM, Judd LM, Boyle KT, Simpson JE, Shull GE, Tanabe H, Ouellette AJ, Franklin CL, Walker NM. Abnormal Paneth cell granule dissolution and compromised resistance to bacterial colonization in the intestine of CF mice. Am J Physiol Gastrointest Liver Physiol. 2004 Jun;286(6):G1050-8. Epub 2004 Jan 8.

Curran EM, Judy BM, Newton LG, Lubahn DB, Rottinghaus GS, MacDonald RS, Franklin CL, Estes DM. Dietary soy phytoestrogens and ERalpha signaling modulate interferon gamma production to bacterial infection. Clin. Exp. Immunol.. 135:219-25, 2004

Gawenis LR, Franklin CL, Simpson JE, Palmer BA, Walker NM, Wiggins TM, Clarke LL. cAMP inhibition of murine intestinal Na/H exchange requires CFTR-mediated cell shrinkage of villus epithelium. Gastroenterology. 2003 Oct;125(4):1148-63.

Alworth LC, Howdeshell KL, Ruhlen RL, Day JK, Lubahn DB, Huang TH, Besch-Williford CL, vom Saal FS. Uterine responsiveness to estradiol and DNA methylation are altered by fetal exposure to diethylstilbestrol and methoxychlor in CD-1 mice: effects of low versus high doses. Toxicol Appl Pharmacol. 2002 Aug 15;183(1):10-22

Carter DB, Lai L, Park KW, Samuel M, Lattimer JC, Jordan KR, Estes DM, Besch-Williford C, Prather RS. Phenotyping of transgenic cloned piglets. Cloning Stem Cells. 2002;4(2):131-45.

Day JK, Bauer AM, DesBordes C, Zhuang Y, Kim BE, Newton LG, Nehra V, Forsee KM, MacDonald RS, Besch-Williford C, Huang TH, Lubahn DB. Genistein alters methylation patterns in mice. J Nutr. 2002 Aug;132(8 Suppl):2419S-2423S.

Phillips CL, Pfeiffer BJ, Luger AM, Franklin CL. Novel collagen glomerulopathy in a homotrimeric type I collagen mouse (oim). Kidney Int. 2002 Aug;62(2):383-91.

Day JK, Besch-Williford C, McMann TR, Hufford MG, Lubahn DB, MacDonald RS. Dietary genistein increased DMBA-induced mammary adenocarcinoma in wild-type, but not ER alpha KO, mice. Nutr Cancer. 2001;39(2):226-32.

Spindler RE, Crichton EG, Agca Y, Loskutoff N, Critser JK, Gardner DK, Wildt DE. Improved felid embryo development by group culture is maintained with heterospecific companions. Theriogenology. 2006 Jul 1;66(1):82-92. Epub 2006 Apr 27.

Lu Q, Zhao Y, Gao X, Li Y, Ma S, Mullen S, Critser JK, Chen ZJ. Combination of calcium ionophore A23187 with puromycin salvages human unfertilized oocytes after ICSI. Eur J Obstet Gynecol Reprod Biol. 2006 May 1;126(1):72-6. Epub 2005 Dec 13.

Jiao A, Han X, Critser JK, Ma H. Numerical investigations of transient heat transfer characteristics and vitrification tendencies in ultra-fast cell cooling processes. Cryobiology. 2006 Apr 5

Li Y, Feng HL, Cao YJ, Zheng GJ, Yang Y, Mullen S, Critser JK, Chen ZJ. Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro. Fertil Steril. 2006 Apr;85(4):827-32.

Benson JD, Haidekker MA, Benson CM, Critser JK. Mercury free operation of the Coulter counter MultiSizer II sampling stand. Cryobiology. 2005 Dec;51(3):344-7. Epub 2005 Sep 27.

Men H, Agca Y, Critser ES, Critser JK. Beneficial effects of serum supplementation during in vitro production of porcine embryos on their ability to survive cryopreservation by open pulled straw vitrification. Theriogenology. 2005 Oct 1;64(6):1340-9. Epub 2005 Apr 12

Men H, Agca Y, Mullen SF, Critser ES, Critser JK. Osmotic tolerance of in vitro produced porcine blastocysts assessed by their morphological integrity and cellular actin filament organization. Cryobiology. 2005 Oct;51(2):119-29.

Agca Y, Mullen S, Liu J, Johnson-Ward J, Gould K, Chan A, Critser J. Osmotic tolerance and membrane permeability characteristics of rhesus monkey (Macaca mulatta) spermatozoa. Cryobiology. 2005 Aug;51(1):1-14.

Agca Y, Liu J, Mullen S, Johnson-Ward J, Gould K, Chan A, Critser J. Chimpanzee (Pan troglodytes) spermatozoa osmotic tolerance and cryoprotectant permeability characteristics. J Androl. 2005 Jul-Aug;26(4):470-7.

Benson JD, Chicone CC, Critser JK. Exact solutions of a two parameter flux model and cryobiological applications. Cryobiology. 2005 Jun;50(3):308-16.

Walters EM, Men H, Agca Y, Mullen SF, Critser ES, Critser JK. Osmotic tolerance of mouse spermatozoa from various genetic backgrounds: acrosome integrity, membrane integrity, and maintenance of motility. Cryobiology. 2005 Apr;50(2):193-205.

Lakey JR, Woods EJ, Zieger MA, Avila JG, Geary WA, Voytik-Harbin SL, Critser JK. Improved islet survival and in vitro function using solubilized small intestinal submucosa. Cell Tissue Bank. 2001;2(4):217-24.

Woods EJ, Walsh CM, Sidner RA, Zieger MA, Lakey JR, Ricordi C, Critser JK. Improved in vitro function of islets using small intestinal submucosa. Transplant Proc. 2004 May;36(4):1175-7.

Woods EJ, Walsh CM, Sidner RA, Zieger MA, Mullin S, Lakey JR, Ricordi C, Critser JK. Enhanced recovery of cryopreserved islets using SIS. Transplant Proc. 2004 May;36(4):1139-42.

Woods EJ, Benson JD, Agca Y, Critser JK. Fundamental cryobiology of reproductive cells and tissues. Cryobiology. 2004 Apr;48(2):146-56. Review.

Mullen SF, Agca Y, Broermann DC, Jenkins CL, Johnson CA, Critser JK. The effect of osmotic stress on the metaphase II spindle of human oocytes, and the relevance to cryopreservation. Hum Reprod. 2004 May;19(5):1148-54. Epub 2004 Apr 7.

Mullen SF, Critser JK. Using TUNEL in combination with an active caspase-3 immunoassay to identify cells undergoing apoptosis in preimplantation Mammalian embryos. Methods Mol Biol. 2004;254:393-406

Mullen SF, Critser JK. Immunocytochemical staining of the metaphase II spindle in mammalian oocytes. Methods Mol Biol. 2004;253:201-13.

Chaveiro A, Liu J, Mullen S, Woelders H, Critser JK. Determination of bull sperm membrane permeability to water and cryoprotectants using a concentration-dependent self-quenching fluorophore. Cryobiology. 2004 Feb;48(1):72-80.

Woods EJ, Liu J, Pollok K, Hartwell J, Smith FO, Williams DA, Yoder MC, Critser JK. A theoretically optimized method for cord blood stem cell cryopreservation. J Hematother Stem Cell Res. 2003 Jun;12(3):341-50.

Guthrie HD, Liu J, Critser JK. Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biol Reprod. 2002 Dec;67(6):1811-6.

Agca Y, Gilmore J, Byers M, Woods EJ, Liu J, Critser JK. Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars. Biol Reprod. 2002 Nov;67(5):1493-501.

Liu J, Christian JA, Critser JK. Canine RBC osmotic tolerance and membrane permeability. Cryobiology. 2002 Jun;44(3):258-68.

Agca Y, Critser JK. Cryopreservation of spermatozoa in assisted reproduction. Semin Reprod Med. 2002 Feb;20(1):15-23.

Keskintepe L, Agca Y, Pacholczyk GA, Machnicka A, Critser JK. Use of cryopreserved pronuclear embryos for the production of transgenic mice. Biol Reprod. 2001 Aug;65(2):407-11.

Bauer BA, Riley LK. Antemortem detection of mouse parvovirus and mice minute virus by polymerase chain reaction (PCR) of faecal samples. Lab Anim. 2006 Apr;40(2):144-52.

Wan CH, Bauer BA, Pintel DJ, Riley LK. Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction. Lab Anim. 2006 Jan;40(1):63-9.

Hsu CC, Wobus CE, Steffen EK, Riley LK, Livingston RS. Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice. Clin Diagn Lab Immunol. 2005 Oct;12(10):1145-51.

Gupta GD, Lakritz J, Saville WJ, Livingston RS, Dubey JP, Middleton JR, Marsh AE. Antigenic evaluation of a recombinant baculovirus-expressed Sarcocystis neurona SAG1 antigen. J Parasitol. 2004 Oct;90(5):1027-33.

Bauer BA, Besch-Williford CL, Riley LK. Comparison of the mouse antibody production (MAP) assay and polymerase chain reaction (PCR) assays for the detection of viral contaminants. Biologicals. 2004 Dec;32(4):177-82.

Livingston, RS, L.K. Riley. Diagnostic testing of mouse and rat colonies for infectious agents. Lab. Animal. 2003 May: 32(5):44-51.

Kendall LV, Riley LK. Flow cytometry. Contemp Top Lab Anim Sci. 2002 Sep;41(5):50.

Livingston RS, Besselsen DG, Steffen EK, Besch-Williford CL, Franklin CL, Riley LK. Serodiagnosis of mice minute virus and mouse parvovirus infections in mice by enzyme-linked immunosorbent assay with baculovirus-expressed recombinant VP2 proteins. Clin Diagn Lab Immunol. 2002 Sep;9(5):1025-31.

Drazenovich NL, Franklin CL, Livingston RS, Besselsen DG. Detection of rodent Helicobacter spp. by use of fluorogenic nuclease polymerase chain reaction assays. Comp Med. 2002 Aug;52(4):347-53.

Wan CH, Soderlund-Venermo M, Pintel DJ, Riley LK. Molecular characterization of three newly recognized rat parvoviruses. J Gen Virol. 2002 Aug;83(Pt 8):2075-83.

Kendall LV, Franklin CL, Riley LK. Immunohistochemistry. Contemp Top Lab Anim Sci. 2001 May;40(3):43

Carty AJ, Franklin CL, Riley LK, Besch-Williford CL. Diagnostic polymerase chain reaction assays for identification of murine polyomaviruses in biological samples. Comp Med. 2001 Apr;51(2):145-9.

MFI Publications by RADIL

Hsu, CC Franklin CL, and Riley LK.  Multiplex Fluorescent Immunoassay.  Lab Animal. 7; 27-30, 2007.

Hsu, CC Franklin CL, and Riley LK.  Multiplex Immunoassay for the Simultaneous Detection of Serum Antibodies to Muliple Rodent Pathogens.  Lab Animal Europe, 7:27-30, 2007.

Tippens AS, Davis SV, Hayes JR, Bryda EC, Green TL, Gruetter CA. Detection of histidine decarboxylase in rat aorta and cultured rat aortic smooth muscle cells. Inflamm Res. 2004 Aug;53(8):390-5. Epub 2004 Aug 10.

Walker E, Black J, Parris C, Bryda EC, Cansino S, Hunt L, Chappell J, Wehner P, Studeny M, Wright GL. Effect of experimental hyperhomocysteinemia on cardiac structure and function in the rat. Ann Clin Lab Sci. 2004 Spring;34(2):175-80

Cogswell C, Price SJ, Hou X, Guay-Woodford LM, Flaherty L, Bryda EC. Positional cloning of jcpk/bpk locus of the mouse. Mamm Genome. 2003 Apr;14(4):242-9.

Price SJ, Chittenden LR, Flaherty L, O'Dell B, Guay-Woodford LM, Stubbs L, Bryda EC. Characterization of the region containing the jcpk PKD gene on mouse Chromosome 10. Cytogenet Genome Res. 2002;98(1):61-6.

Chittenden L, Lu X, Cacheiro NL, Cain KT, Generoso W, Bryda EC, Stubbs L. A new mouse model for autosomal recessive polycystic kidney disease. Genomics. 2002 Apr;79(4):499-504.

Bryda EC, Kim HJ, Legare ME, Frankel WN, Noben-Trauth K. High-resolution genetic and physical mapping of modifier-of-deafwaddler (mdfw) and Waltzer (Cdh23v). Genomics. 2001 May 1;73(3):338-42.

Bolz H, von Brederlow B, Ramirez A, Bryda EC, Kutsche K, Nothwang HG, Seeliger M, del C-Salcedo Cabrera M, Vila MC, Molina OP, Gal A, Kubisch C. Mutation of CDH23, encoding a new member of the cadherin gene family, causes Usher syndrome type 1D. Nat Genet. 2001 Jan;27(1):108-12.

Di Palma F, Holme RH, Bryda EC, Belyantseva IA, Pellegrino R, Kachar B, Steel KP, Noben-Trauth K. Mutations in Cdh23, encoding a new type of cadherin, cause stereocilia disorganization in waltzer, the mouse model for Usher syndrome type 1D. Nat Genet. 2001 Jan;27(1):103-7.

Mock OB, Casteel SW, Darmani NA, Shaddy JH, Besch-Williford C, Towns LC. 1,3-dinitrobenzene toxicity in the least shrew, Cryptotis parva. Environ Toxicol Chem. 2005 Oct;24(10):2519-25

Hsu C, Carter DB, Williams D, Besch-Williford C. Haematoloechus sp. infection in wild-caught northern leopard frogs (Rana pipiens). Contemp Top Lab Anim Sci. 2004 Nov;43(6):14-6; quiz 58.

St Claire MB, Kennett MJ, Besch-Williford CL. Vitamin A toxicity and vitamin E deficiency in a rabbit colony. Contemp Top Lab Anim Sci. 2004 Jul;43(4):26-30.

Carter DB, Kennett MJ, Franklin CL. Use of perphenazine to control cannibalism in DBA/1 mice. Comp Med. 2002 Oct;52(5):452-5. Erratum in: Comp Med. 2003 Apr;53(2):218.

Simmons JH, Purdy GA, Franklin CL, Trottier P, Churchill AE, Russell RJ, Besch-Williford CL, Riley LK. Characterization of a novel parainfluenza virus, caviid parainfluenza virus 3, from laboratory guinea pigs (Cavia porcellus). Comp Med. 2002 Dec;52(6):548-54.

Kendall LV, Riley LK, Hook RR Jr, Besch-Williford CL, Franklin CL. Characterization of lymphocyte subsets in the bronchiolar lymph nodes of BALB/c mice infected with cilia-associated respiratory bacillus. Comp Med. 2002 Aug;52(4):322-7.

Myles MH, Foltz CJ, Shinpock SG, Olszewski RE, Franklin CL. Infertility in CFW/R1 mice associated with cystic dilatation of the bulbourethral gland. Comp Med. 2002 Jun;52(3):273-6.

Ramos-Vara, JA, Franklin CL, Miller MA. Bronchitis and bronchiolitis in a cat with CAR bacillus-like organisms and intraepithelial globule leukocytes. Vet Pathol. 39:501-504, 2002

Mock OB, Casteel SW, Darmani NA, Shaddy JH, Besch-Williford C, Towns LC. Anatomic and physiologic reference values in least shrews (Cryptotis parva). Comp Med. 2001 Dec;51(6):534-7.

Criley JM, Carty AJ, Besch-Williford CL, Franklin CL. Coxiella burnetii infection in C.B-17 scid-bg mice xenotransplanted with fetal bovine tissue. Comp Med. 2001 Aug;51(4):357-60.

Simmons JH, Riley LK, Franklin CL, Besch-Williford CL. Hamster polyomavirus infection in a pet Syrian hamster (Mesocricetus auratus). Vet Pathol. 2001 Jul;38(4):441-6.

Kendall LV, Riley LK, Hook RR Jr, Besch-Williford CL, Franklin CL. Differential interleukin-10 and gamma interferon mRNA expression in lungs of cilium-associated respiratory bacillus-infected mice. Infect Immun. 2001 Jun;69(6):3697-702.

Murine Norovirus Publications by RADIL

Hsu CC, Wobus CE, Steffen EK, Riley LK and Livingston, RS.  Development of a Microsphere-Based Serologic Multiplexed Fluorescent Immunoassay and Reverse Transcriptase-PCR Assay to Detect Murine Norovirus 1 (MNV-1) Infection in Mice.  Clin. Diagn. Lab. Immunol.  12:1145-1151, 2005.

Hsu CC, Riley LK, Wills HM and Livingston RS.  Persistent Infection and Serologic Cross-Reactivity of Three Novel Murine Noroviruses.  Comp. Med. 56:247-251, 2006.

Hsu CC, Riley LK and Livingston RS.  Molecular characterization of three noval murine noroviruses.  Virus Genes.  34:147-55, 2007.

Manuel CA, Hsu CC, Riley LK and Livingston RS.  Soiled Bedding Sentinel Detection of Murine Norovirus 4 (MNV-4).  JAALAS 47:31-36, 2008.

Manuel CA, Hsu CC, Riley LK, and Livingston RS.  Soiled Bedding Sentinel Detection of Murine Norovirus 4.  JAALAS 47:31-36, 2008.

Besselsen DG, Romero MJ, Wagner AM, Henderson KS, Livingston RS. Identification of novel murine parvovirus strains by epidemiological analysis of naturally infected mice. J Gen Virol. 2006 Jun;87(Pt 6):1543-56.

Bauer BA, Riley LK. Antemortem detection of mouse parvovirus and mice minute virus by polymerase chain reaction (PCR) of faecal samples. Lab Anim. 2006 Apr;40(2):144-52.

Wan CH, Bauer BA, Pintel DJ, Riley LK. Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction. Lab Anim. 2006 Jan;40(1):63-9.

Livingston RS, Besselsen DG, Steffen EK, Besch-Williford CL, Franklin CL, Riley LK. Serodiagnosis of mice minute virus and mouse parvovirus infections in mice by enzyme-linked immunosorbent assay with baculovirus-expressed recombinant VP2 proteins. Clin Diagn Lab Immunol. 2002 Sep;9(5):1025-31.

Wan CH, Soderlund-Venermo M, Pintel DJ, Riley LK. Molecular characterization of three newly recognized rat parvoviruses. J Gen Virol. 2002 Aug;83(Pt 8):2075-83.