The RADIL professional staff has operated a laboratory animal diagnostic laboratory for over 40 years. Our professional staff includes a wide breadth of expertise including ACLAM-boarded veterinarians and PhD microbiologists, molecular biologists, geneticists and cryobiologists.
January 21st, 2010
In at least two recent articles, major research journals have cited the grim statistics associated with the use of misidentified or cross-contaminated cell lines and their impact on research. The Journal of the National Cancer Institute this week calculated the number of scientific publications (100),cancer research grants (3) and U.S. patents (11) which have now been affected by cell lines used to study drugs for esophageal cancer.
Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and clearly has a substantial negative impact on biomedical research. RADIL offers CellCheckTM for analyzing human and rodent cell lines for inter- and intra-species contamination followed by authentication of the cell line identity. Multiplex PCR analysis using primers specific for mouse, rat, human, African green monkey and Chinese hamster genomic DNA allows confirmation of the species of origin of a given cell line and enables as little as 1-5% inter-species DNA contamination to be detected. Cell lines can be genetically authenticated using microsatellite marker short tandem repeat (STR) analysis which also allows intra-species contamination to be detected. Since offering CellCheckTM cell line testing, RADIL has found that 19% of submitted cell lines were misidentified and at least 4% were cross-contaminated.
Testing of cell lines using RADIL’s CellCheckTM provides a straightforward, quick, reliable, and cost-effective method for detecting cross-contamination of cell lines while verifying their identity. CellCheckTM testing is also available for pig, rabbit and dog. For more information and pricing, please visit our CellCheckTMpage, or contact us at 800-669-0825.
To view our recent scientific poster entitled "Cell Line Authentication Testing" or to listen to or download an .mp3 audio abstract, please visit our Technical Library.
By an overwhelming margin, the most prevalent pathogen that we see in rats is RTV/TMEV (or Rat Theiler Virus). Also known as Rat Theilovirus, TMEV-like virus of rats, and Rat encephalomyelitis virus, RTV is present two to two and a half times more frequently than RCV, RMV, PVM or H-1. RADIL has both an MFI serologic assay as well as a PCR assay with which to test for RTV.
For more detailed information about RTV prevalence, download RTV Prevalency Data excerpted from Infectious Agents in Laboratory Rodents.
November 19th, 2009
RADIL now offers an alternative to direct exam for detecting the presence of pinworms. In the past, direct exam was the most accurate method of detection, however it could only be done post-mortem. With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results. RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation. In studies RADIL's Pinworm PCR test was also more sensitive than either of the two antemortem tests (tape test and fecal float).
Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay. For more information and pricing, please click here.
( for more info click here )November 19th, 2009
At this year's National AALAS Meeting in Denver, Colorado, RADIL 
introduced a breakthrough serologic testing technology that offers clients an increased level of results confidence for the most prevalent mouse and rat agents. MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available. By evaluating multiple antigens for each agent, primary and confirmatory testing now occur simultaneously, saving time and increasing the predictive value of the final results. Clients will begin seeing multiple antigens reported on case reports as of February 1, 2010.
For more information regarding MFI2, please visit the Serology section of this site.
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